Sepsis Flow Chip is an in vitro diagnostic kit for human nosocomial infections based on multiplex PCR and reverse dot blot hybridization for simultaneous detection of bacteria, fungi, and major antibiotic resistance genes in a single assay. Sepsis Flow Chip system allows simultaneous detection of over 36 bacteria species (Coagulase-Negative Staphylococci, Staphylococcus aureus, Streptococcus spp., Streptococcus
pneumoniae, Streptococcus agalactiae, Streptococcus pyogenes, Listeria monocytogenes, Enterococcus spp., Pseudomonas aeruginosa, Acinetobacter baumannii, Neisseria meningitidis, Stenotrophomonas maltophilia, Escherichia coli, Klebsiella pneumoniae, Serratia marcescens, Enterobacteriaceae species, and Proteus/Morganella spp.), fungi species (Candida albicans and Candida spp.) and twenty antibiotic
resistance markers. Regarding the antibiotic resistance markers, the kit detects one gene for methicillin
resistance (mecA), two genes for vancomycin resistance (vanA and vanB), two for β-lactam antibiotic resistance (blaSHV and extended-spectrum blaCTX- M), and fifteen genes for carbapenems resistance (kpc allele: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 and 23, sme allele: 1, 2, 3, 4
and 5, nmc/imi allele: 1, 2, 3, 4, 5, 6, 7, 8 and 9, ges allele: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,
17, 18, 19, 20, 21, 22, 23, 24, 25 and 26, vim allele: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,
19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45 and 46,
gim allele: 1 and 2, spm, ndm allele: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 and 16, sim, imp3, 15,
19_like allele: 1, 2, 3, 5, 6, 8, 9, 10, 11, 15, 19, 20, 21, 24, 25, 28, 29, 30, 40, 41, 42 and 47, oxa23_like
allele: 23, 27, 49, 73, 133, 146, 165, 166, 167, 168, 169, 170, 171 and 225, oxa24_like allele: 24, 25, 26, 40,
72, 139 and 160, oxa48_like allele: 48, 162, 163 and 181, oxa51_like allele: 51, 60, 65, 66, 67, 68, 69, 70,
75, 76, 77, 78, 79, 80, 82, 83, 84, 88, 89, 90, 91, 92, 93, 94, 95, 98, 99, 106, 107, 108, 109, 110, 111, 112,
113, 115, 116, 117, 128, 130, 131, 132, 138, 144, 148, 149, 150, 172, 173, 174, 175, 176, 177, 178, 179,
180, 195, 196, 197, 194, 200, 201, 202, 203, 206, 208 and 223, oxa58_like allele: 58, 96, 97 and 164). The
method is based on amplifying the target DNAs with two multiplex PCR reactions and subsequently
hybridizing the biotinylated amplicons to specific DNA probes.