Description

CFSE Cell Division Tracker Kit is composed of 5 vials, 100 µg per vial of CFSE (formally known
as 5-(and 6)-Carboxyfluorescein diacetate succinimidyl ester of CFDA SE), and 500 µl of
anhydrous DMSO. CFSE is able to passively diffuse into cells. Inside the cell, its acetate
groups are cleaved by intracellular esterases, and the molecules are converted to fluorescent
esters. CFSE is retained within the cell and covalently couples to intracellular molecules via its
succinimidyl group. Due to this covalent coupling reaction, fluorescent CFSE can be retained
within the cell for an extremely long period. Also, due to this stable linkage, once the dye has
been incorporated within the cell, it is not transferred to adjacent cells. CFSE is widely used for
cell proliferation assays and in vivo cell tracking.