HPV Direct Flow CHIP is an in vitro diagnostic kit for the detection of human papillomavirus (HPV). The
infection with HPV is an essential factor in cervical and anogenital carcinogenesis (zur Hausen et al, 1974;
Walboomer et al, 1999; zur Hausen, 1996; zur Hausen 2002).
Based onthe association with different degrees of cervical lesions, the different HPV genotypes have been
classified (Muñoz 2003) as high-risk or oncogenic genotypes, which can induce carcinogenesis; and low-risk
HPVs, which cause genital warts and collaborate with high-risk HPVs.
The HPV Direct Flow CHIP allows the qualitative detection of the HPV and the genotyping of 35 types of
HPV (high-risk HPV 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73 and 82, and low-risk HPV
6, 11, 40, 42, 43, 44, 54, 55, 61, 62, 67, 69, 70, 71, 72, 81 and 84) by PCR (polymerase chain reaction),
followed by reverse hybridization on a membrane containing specific probes. This system accepts both
clinical samples that can be amplified directly, without the need for prior DNA extraction, and the
amplification product obtained with the Vitro HPV Screening kit (Vitro, S.A. Ref. MAD-003949M).
Microbiological status: Non-sterile product.