The Respiplex (RSV-MPV-RhV) RT-PCR kit is an in vitro diagnostic kit for the simultaneous qualitative detection and differentiation of RNA of the Respiratory Syncytial Virus1, Metapneumovirus (MPV) and/or Rhinovirus (RHV) from RNA extracted from human clinical samples of different origin such as nasopharyngeal and oropharyngeal exudates and bronchoalveolar lavages (BAL). It can be used simultaneously with the FLU-COVID RT-PCR kit for the detection of Influenza A (FluA), Influenza B (FluB) and/or SARS-CoV-2 in a complementary manner.
It is based on the multiplex One-Step RT-PCR technique, using primers and fluorescent probes for the target genes: matrix protein gene (M) of RSV, nucleoprotein gene (N) of MPV and 5’ region UTR of RhV.
1Types of Respiratory Syncytial Viruses detected: A and B.
Specific primers and fluorescent probe are included for the simultaneous detection of the human RNaseP gene as internal quality control of the starting and amplification material. The detection channels of the different targets are: